Dopamine b-Hydroxylase Antibody

• Background:

  Dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. The expression of DBH is elevated during stress.

• NCBI Gene ID:

  1621

• Swiss Prot:

  P09172

• Accession Number:

  P09172

• Host:

  Sheep

• Reactivity:

  Human, Rat, Sheep, Bovine

• Immunogen:

  DBH polyclonal antibody was raised against a synthetic peptide from the N-terminal region of human DBH, conjugated to keyhole limpet hemocyanin.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

• Calculated Molecular Weight:

  75

• Specificity:

  Dopamine b-Hydroxylase, NT antibody is specific immunolabeling of the ~75k DBH protein in Western blots of human adrenal medulla.

• Applications Notes:

  Applications include Dot Blots (DB) and Western Blots (WB) . Sheep anti-DBH recognizes DBH in bovine, human, monkey and rat tissues. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

• Symbol:

  DBH

• NCBI Official Name:

  Dopamine beta-hydroxylase (dopamine beta-monooxygenase)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sabban, E.L., Kvetnansky, R. "Stress-triggered activation of gene expression in catecholaminergic systems: dynamics of transcriptional events, " Trends Neurosci. 24 (2001) 91 - 98.

• Background Reference 02:

  Nagatsu, I, Kobayashi, K, Fujii, T, Komori, K, Sekiguchi, K, Titani, K, Fujita, K, and Nagatsu, T. "Antibodies raised against different oligopeptide segments of human dopamine-β -hydroxylase, " Neuroscience Letters, 120 (1990) 141 - 145.

• Tested Applications:

  WB

• Protein ID:

  158517849

• Physical Properties:

  Liquid