Dopamine Decarboxylase Antibody

• Background:

  Aromatic L-amino acid decarboxylase (AADC; DOPA decarboxylase; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin. It is also involved in the biosynthesis of trace amines.

• NCBI Gene ID:

  280762

• Swiss Prot:

  P27718

• Accession Number:

  P27718

• Host:

  Rabbit

• Reactivity:

  Rat, Bovine

• Immunogen:

  DOPA Decarboxylase polyclonal antibody was raised against a SDS denatured, recombinant bovine aromatic DDC expressed in E. coli and purified from inclusion bodies.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

• Calculated Molecular Weight:

  55

• Specificity:

  Dopamine Decarboxylase antibody is specific immunolabeling of the ~55k DDC protein in Western blots of rat adrenal medulla.

• Applications Notes:

  Applications include Dot Blots (DB) and Western Blots (WB) . Recognizes DDC in rat adrenal medulla and caudate. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

• Symbol:

  DDC

• NCBI Official Name:

  Dopa decarboxylase (aromatic L-amino acid decarboxylase)

• NCBI Organism:

  Bos taurus

• Background Reference 01:

  Haycock, J.W., Becker, L., Ang, L., Furukawa, Y., Hornykiewicz, O., and Kish, S.J., "Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum, " J. Neurochem. 87 (2003) 574 - 585.

• Background Reference 02:

  Waymire, J.C. and Haycock, J.W. "Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells, " J. Neurochem. 81 (2002) 589 - 593.

• Background Reference 03:

  Berry, M.D., Juorio, A.V., Li, X.M., and Boulton, A.A. "Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme, " Neurochem. Res. 21 (1996) 1075 -1087.

• Tested Applications:

  WB

• Protein ID:

  125987819

• Physical Properties:

  Liquid