Clusterin Antibody [CLI-9]

• NCBI Gene ID :

  1191

• Swiss Prot :

  P10909

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Clusterin (monoclonal CLI-9) antibody was raised against human clusterin (TRPM-2) .

• Clonality :

  Monoclonal

• Clone :

  CLI-9

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Apoptosis, Neuroscience

• Purification :

  Clusterin (monoclonal CLI-9) antibody was purified to ≥95% in SDS-PAGE.

• Concentration :

  Batch dependent

• Buffer :

  PBS containing 0.02% sodium azide

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Clusterin antibody can be stored at 4˚ C, stable for one year.

• Fragment :

  IgG1

• Applications Notes :

  Clusterin antibody can be used for detection of human clusterin by immunocytochemistry, immunohistochemistry (frozen sections), immunoprecipitation, and western blot (1:2,000) . Method: : Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2,000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.

• NCBI Official Name :

  Clusterin

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Large-scale serial analysis of gene expression reveals genes differentially expressed in ovarian cancer: C. D. Hough, et al.; Cancer Res. 60, 6281 (2000) .

• Background Reference 02 :

  Characterization and cloning of androgen-repressed mRNAs from rat ventral prostate:J.G. Leger, et al.; BBRC 147, 196 (1987) .

• Background Reference 03 :

  Apolipoprotein J: structure and tissue distribution: H.V. De Silva, et al.; Biochemistry 29, 5380 (1990) .

• Background Reference 04 :

  Clusterin: the intriguing guises of a widely expressed glycoprotein: D.E. Jenne & J. Tschopp; Trends Biochem. Sci. 17, 154 (1992) .

• Tested Applications :

  WB, IHC, ICC, IP

• Physical Properties :

  Liquid