Clusterin Antibody [CLI-9]

• NCBI Gene ID:

  1191

• Swiss Prot:

  P10909

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Clusterin (monoclonal CLI-9) antibody was raised against human clusterin (TRPM-2) .

• Clonality:

  Monoclonal

• Clone:

  CLI-9

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Neuroscience

• Purification:

  Clusterin (monoclonal CLI-9) antibody was purified to ≥95% in SDS-PAGE.

• Concentration:

  Batch dependent

• Buffer:

  PBS containing 0.02% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Clusterin antibody can be stored at 4˚ C, stable for one year.

• Fragment:

  IgG1

• Applications Notes:

  Clusterin antibody can be used for detection of human clusterin by immunocytochemistry, immunohistochemistry (frozen sections), immunoprecipitation, and western blot (1:2,000) . Method: : Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2,000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.

• NCBI Official Name:

  Clusterin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Large-scale serial analysis of gene expression reveals genes differentially expressed in ovarian cancer: C. D. Hough, et al.; Cancer Res. 60, 6281 (2000) .

• Background Reference 02:

  Characterization and cloning of androgen-repressed mRNAs from rat ventral prostate:J.G. Leger, et al.; BBRC 147, 196 (1987) .

• Background Reference 03:

  Apolipoprotein J: structure and tissue distribution: H.V. De Silva, et al.; Biochemistry 29, 5380 (1990) .

• Background Reference 04:

  Clusterin: the intriguing guises of a widely expressed glycoprotein: D.E. Jenne & J. Tschopp; Trends Biochem. Sci. 17, 154 (1992) .

• Tested Applications:

  WB, IHC, ICC, IP

• Physical Properties:

  Liquid