Clusterin Recombinant Protein

• Background:

  Clusterin (CLU) is also known as dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI), is a secreted multifunctional glycoprotein protein which belongs to the clusterin family. Intracellular cleavages of the precursor of clusterin remove the signal peptide and generate comparably sized α and β chains which are secreted as an 80 kDa N­glycosylated disulfide­linked heterodimer. DAG protein is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development. Clusterin involve in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.

• NCBI Gene ID:

  1191

• Swiss Prot:

  P10909

• Accession Number:

  NP_001822.3

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Apoptosis, Cancer, Homeostasis, Immunology, Innate Immunity, Neuroscience, Obesity

• Purity:

  >92% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  50.9 kDa (Disulfide - linked heterodimer)

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Clusterin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jones SE., et al., Int. J. Biochem. Cell Biol. 34 (5) : 427–431.

• Background Reference 02:

  Poon S., et al., 2002, J. Biol. Chem. 277:39532-39540.

• Background Reference 03:

  Leskov K.S., et al., 2003, J. Biol. Chem. 278:11590-11600.

• Background Reference 04:

  Santilli G., et al., 2003, J. Biol. Chem. 278:38214-38219.

• Other Product Names:

  CLU, Clusterin, AAG4, APOJ, CLI, KUB1, SGP-2, SP-40, TRPM2

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3