PUMA Antibody [10D4G7]

• Background:

  PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

• NCBI Gene ID:

  27113

• Swiss Prot:

  Q9BXH1

• Accession Number:

  Q9BXH1

• Host:

  Mouse

• Reactivity:

  Human, Rat

• Immunogen:

  Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.

• Clonality:

  Monoclonal

• Clone:

  10D4G7

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  PUMA Monoclonal Antibody is Protein A purified.

• Positive Control:

  Cat. No. 1204 - K562 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PUMA Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PUMA monoclonal antibody can be stored at -20˚ C, stable for one year.

• Fragment:

  IgG1

• Symbol:

  BBC3

• Positive Control 2:

  Cat. No. 95-102 - PUMA Recombinant Protein

• NCBI Official Name:

  BCL2 binding component 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.

• Background Reference 02:

  Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.

• Background Reference 03:

  Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.

• Other Product Names:

  PUMA Antibody [10D4G7] : JFY1, PUMA, JFY-1

• Tested Applications:

  ELISA, WB

• Protein ID:

  56748610

• Physical Properties:

  Liquid