PUMA Antibody

• Background:

  PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1) . The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2) . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

• NCBI Gene ID:

  27113

• Swiss Prot:

  Q96PG8

• Accession Number:

  NP_055232

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  3043

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation (Figure 2) shows similar PUMA expression profile in both human and mouse cell lines detected by two independent anti-PUMA antibodies that recognize different epitopes, 3041 against C-terminus and 3043 against the N-terminus. PUMA proteins are detected in most of the cell lines with different expression levels by the two independent antibodies. Independent Antibody Validation3041 3043 KO Validation (Figure 7, 8) shows lack of PUMA expression in the thymocytes or neurons of PUMA knockout mice. KO Validation Animal Species Reactivity (Figure 10) shows PUMA expression in the rat retina detected with anti-PUMA antibodies (3043) . Animal Species Reactivity

• Field of Research:

  Apoptosis, Autophagy, Cancer

• Purification:

  PUMA Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1210 - 293 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (78.6%)

• Buffer:

  PUMA Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PUMA antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD), isoform 2 (131aa, 14kD), isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD) . This antibody detects human isoform 1, and is predicted to detect mouse and rat PUMA (193aa, 21kD for both of them) .

• Symbol:

  BBC3

• Positive Control 2:

  Cat. No. 1211 - HepG2 Cell Lysate

• Positive Control 3:

  Cat. No. 10-001 - Human Breast Tissue Slide

• Positive Control 4:

  Cat. No. 1204 – K562 Cell Lysate

• NCBI Official Name:

  BCL2 binding component 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Nakano and Vousden. Mol Cell. 2001; 7 (3) 683-94

• Background Reference 02:

  Han et al. Proc Natl Acad Sci U S A. 2001;98 (20) :11318-23.

• Citation 01:

  18259198

• Citation 02:

  23056511

• Citation 03:

  18604177

• Citation 04:

  22736709

• Citation 05:

  21658600

• Citation 06:

  29686375

• Citation 07:

  27655665

• Citation 08:

  30068931

• Other Product Names:

  PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  15193488

• Physical Properties:

  Liquid