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PUMA Antibody

CAT: 0223-3041-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-3041-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1) . The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2) . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
NCBI Gene ID
27113
Swiss Prot
Q96PG8
Accession Number
NP_055232
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
3041
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation (Figure 2) shows similar PUMA expression profile in both human and mouse cell lines detected by two independent anti-PUMA antibodies that recognize different epitopes, 3041 against C-terminus and 3043 against the N-terminus. PUMA proteins are detected in most of the cell lines with different expression levels by the two independent antibodies. Independent Antibody Validation3041 3043 siRNA Knockdown Validation (Figure 3) : Anti-PUMA antibody (3041) specificity was further verified by PUMA specific siRNA knockdown. PUMA signal in HeLa cells transfected with PUMA siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA Knockdown Validation3041
Field of Research
Apoptosis, Neuroscience, Autophagy, Cancer
Purification
PUMA Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1210 - 293 Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Buffer
PUMA Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
PUMA antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
23 kDa
Fragment
IgG
Specificity
A lower band at approximately 16 kDa was detected in Daudi and K562 cells, which may represent the PUMA isoform 2.
Isoform
Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD), isoform 2 (131aa, 14kD), isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD) . This antibody detects human isoform 1&2, mouse and rat PUMA (193aa, 21kD for both of them) .
Symbol
BBC3
Positive Control 2
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 3
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 4
Cat. No. 1204 - K562 Cell Lysate
NCBI Official Name
BCL2 binding component 3
NCBI Organism
Homo sapiens
Background Reference 01
Nakano and Vousden. Mol Cell. 2001; 7 (3) 683-94
Background Reference 02
Han et al. Proc Natl Acad Sci U S A. 2001;98 (20) :11318-23.
Citation 01
21353614
Citation 02
20404322
Citation 03
25057207
Citation 04
27056669
Citation 05
26470728
Citation 06
26247726
Citation 07
17297446
Citation 08
28427210
Other Product Names
PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3
Tested Applications
ELISA, WB, ICC, IF
Protein ID
15193488
Physical Properties
Liquid

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