HSF1 (phospho Thr142) Antibody

• Background:

  DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

• NCBI Gene ID:

  3297

• Swiss Prot:

  Q00613

• Accession Number:

  NP_005517.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  HSF1 (phospho Thr142) antibody was raised against a peptide sequence around phosphorylation site of threonine 142 (L-L-T (p) -D-V) derived from Human HSF1 .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  57 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of HSF1 only when phosphorylated at threonine 142.

• Symbol:

  HSF1

• NCBI Official Name:

  Heat shock transcription factor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rabindran S.K., Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910 (1991) .

• Background Reference 02:

  The MGC Project Team; Genome Res. 14:2121-2127 (2004) .

• Background Reference 03:

  Schuetz T.J., Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915 (1991)

• Other Product Names:

  HSF 1, HSTF1, Heat shock factor protein 1

• Tested Applications:

  IHC

• Protein ID:

  5031767

• Physical Properties:

  Liquid