HSF1 (Phospho-Thr142) Antibody

• Background:

  DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

• Description:

  This is a HSF1 (Phospho-Thr142) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  HSF 1; HSTF1; Heat shock factor protein 1

• Gene ID:

  3297

• Swiss Prot:

  Q00613

• Reactivity:

  Human, Mouse

• Immunogen:

  Peptide sequence around phosphorylation site of threonine 142 (L-L-T (p) -D-V) derived from Human HSF1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  IHC 1:50-1:100

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Observed Molecular Weight:

  57kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG