Cyclin H (phospho Thr315) Antibody

• Background:

  Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

• NCBI Gene ID:

  902

• Swiss Prot:

  P51946

• Accession Number:

  NP_001230.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Cyclin H (phospho Thr315) antibody was raised against a peptide sequence around phosphorylation site of threonine 315 (E-W-T (p) -D-D) derived from Human Cyclin H .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  36 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of Cyclin H only when phosphorylated at threonine 315.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  CCNH

• NCBI Official Name:

  Cyclin H

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Maekelae T.P., Nature 371:254-257 (1994) .

• Background Reference 02:

  Fisher R.P., Cell 78:713-724 (1994) .

• Background Reference 03:

  The MGC Project Team; Genome Res. 14:2121-2127 (2004) .

• Other Product Names:

  Cyclin H, p34, p37, MO15-associated protein

• Tested Applications:

  WB

• Protein ID:

  4502623

• Physical Properties:

  Liquid