Cyclin B1 (phospho Ser147) Antibody

• Background:

  The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

• NCBI Gene ID:

  891

• Swiss Prot:

  P14635

• Accession Number:

  NP_114172.1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Cyclin B1 (phospho-Ser147) antibody was raised against a peptide sequence around phosphorylation site of Serine 147 (A-F-S (p) -D-V) derived from Human Cyclin B1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  60 kDa

• Specificity:

  This antibody detects endogenous level of Cyclin B1 only when phosphorylated at serine 147.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  CCNB1

• NCBI Official Name:

  Cyclin B1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.

• Other Product Names:

  CCNB, VEGFR3

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  14327896

• Physical Properties:

  Liquid