PKA alpha/beta CAT (phospho Thr197) Antibody

• Background:

  Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.

• NCBI Gene ID:

  5566

• Swiss Prot:

  P17612

• Accession Number:

  NP_002721.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  PKA alpha/beta CAT (phospho Thr197) antibody was raised against a peptide sequence around phosphorylation site of threonine 197 (R-T-W (p) -T-L) derived from Human PKA alpha/beta CAT.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  40 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  PRKACA

• NCBI Official Name:

  Protein kinase, cAMP-dependent, catalytic, alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259.

• Background Reference 02:

  W Li, Learn. Mem., Jan 1996; 2: 320 - 333.

• Background Reference 03:

  Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.

• Background Reference 04:

  Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.

• Other Product Names:

  PRKACA , PRKACB, PKACA

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  4506055

• Physical Properties:

  Liquid