Twist1 Antibody

• Background:

  Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

• NCBI Gene ID:

  7291

• Swiss Prot:

  Q15672

• Accession Number:

  NP_000465.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Twist1 antibody was raised against a peptide sequence around aa.18~22 (S-N-S-E-E) derived from Human Twist1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  28 kDa

• Specificity:

  This antibody detects endogenous levels of total Twist1 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  TWIST1

• NCBI Official Name:

  Twist family bHLH transcription factor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  "Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity. "Sosic D., Richardson J. A., Yu K., Ornitz D. M., Olson E. N. Cell 112:169-180 (2003) "Mutations of the TWIST gene in the Saethre-Chotzen syndrome. "el Ghouzzi V., le Merrer M., Perrin-Schmitt F., et al., Nat. Genet. 15:42-46 (1997)

• Other Product Names:

  CRS, SCS, ACS3, CRS1, BPES2, BPES3, TWIST, bHLHa38

• Tested Applications:

  WB

• Protein ID:

  4507741

• Physical Properties:

  Liquid