TIF1beta Antibody

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs) . Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me) ) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21 (CIP1) . Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.

10155

Q13263

NP_005753.1

Human, Mouse, Rat

TIF1beta antibody was raised against a peptide sequence around aa.827~831 (L-S-G-G-P) derived from Human TIF1β .

Unconjugated

Primary Antibodies

Cell Cycle, Stem Cell

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

None

Blue Ice

Store antibody at -20˚ C for up to one year.

110 kDa

This antibody detects endogenous level of total TIF1β protein.

Western Blot: 1:500~1:1000

TRIM28

Tripartite motif containing 28

Homo sapiens

Friedman J. R., Fredericks W. J., Jensen D. E., Speicher D. W., Huang X. -P., Neilson E. G., Rauscher F. J. IIIGenes Dev. 10:2067-2078 (1996) Moosmann P. R., Georgiev O., le Douarin B., Bourquin J. -P., Schaffner W. Nucleic Acids Res. 24:4859-4867 (1996) Agata Y., Matsuda E., Shimizu A. J. Biol. Chem. 274:16412-16422 (1999)

KAP1, TF1B, RNF96, TIF1B, KAP1

WB

5032179