BRCA1 (Ab 1524) Antibody

• Background:

  The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

• NCBI Gene ID:

  672

• Swiss Prot:

  P38398

• Accession Number:

  NP_009225.1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  BRCA1 (Ab-1524) antibody was raised against a peptide sequence around aa.1522~1526 (Y-P-S-Q-E) derived from Human BRCA1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  220 kDa

• Specificity:

  This antibody detects endogenous level of total BRCA1 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

• Symbol:

  BRCA1

• NCBI Official Name:

  Breast cancer 1, early onset

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lin SY, et al. (2004) Proc Natl Acad Sci U S A, 101 (17) : 6484-6489Zhang J, et al. (2004) Mol Cell Biol; 24 (2) : 708-718Beger C, et al. (2001) Proc Natl Acad Sci U S A; 98 (1) : 130-135Gardner K, et al. (2001) Breast Cancer Res; 3 (1) : 11-13Zheng L, et al. (2001) Proc Natl Acad Sci U S A; 98 (17) : 9587-9592

• Other Product Names:

  IRIS, PSCP, BRCAI, BRCC1, PNCA4, RNF53, BROVCA1, PPP1R53, ASK1, MAPKKK5, MEKK5

• Tested Applications:

  WB, IF

• Protein ID:

  6552299

• Physical Properties:

  Liquid