BRCA1 Antibody

• Background:

  This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC) . This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

• NCBI Gene ID:

  672

• Swiss Prot:

  P38398

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A synthetic peptide corresponding to a sequence within amino acids 120-220 of human BRCA1 (NP_009225.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  Affinity purification

• Positive Control:

  HeLa

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 85kDa

• Fragment:

  IgG

• Symbol:

  BRCA1

• Positive Control 2:

  MCF-7

• Positive Control 3:

  HL-60

• Positive Control 4:

  HepG2

• NCBI Official Name:

  Breast cancer 1, early onset

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Breast cancer type 1 susceptibility protein, 632-, RING finger protein 53, BRCA1, RNF53

• Tested Applications:

  WB, IHC, IF

• Physical Properties:

  Liquid