Dynamin (phospho Ser778) Antibody

• Background:

  Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.

• NCBI Gene ID:

  140694

• Swiss Prot:

  P21575

• Accession Number:

  P21575

• Host:

  Sheep

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Dynamin (Ser778) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser778 of human, mouse and rat dynamin.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Phospho-Specific

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For long term storage -80˚ C is recommended, but shorter term storage at -20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚ C.

• Calculated Molecular Weight:

  95

• Applications Notes:

  Dynamin antibody for phospho-Ser778 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003) . The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser778) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin) . Applications include Western Blots (WB) . Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for WB.

• Symbol:

  Dnm1

• NCBI Official Name:

  Dynamin 1

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Tan, T.C., Valova, V.A., Malladi, C.S., Graham, M.E., Berven, L.A., Jupp, O.J., Hansra, G, McClure, S.J., Surcevic, B, Boadle, R.A., Larsen, M.R., Cousin, M.A., Robinson, P.J., "Cdk5 is essential for synaptic vesicle endocyotosis, " Nat. Cell Biol., 8 (2003) 701-710.

• Background Reference 02:

  Graham, M.E., O'Callaghan, D.W., McMahon, H.T., and Burgoyne, R.D., "Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size, " Proc. Natl. Acad. Sci. USA, 99 (2002) 7124 - 7129.

• Background Reference 03:

  Tsuboi, T., Terakawa, S., Scalettar, B.A., Fantus, C., Roder, J., and Jeromin, A., "Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins, " J. Biol. Chem., 277 (2002) 15,957-15,961.

• Background Reference 04:

  Cousin, M.A., Tan, T.C., and Robinson, P.J., "Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin, " J. Neurochem., 76 (2001) 105-116.

• Tested Applications:

  WB

• Protein ID:

  190358918

• Physical Properties:

  Liquid