CD357 Antibody [DTA-1] (APC)

• Background:

  The DTA-1 monoclonal antibody specifically reacts with mouse Glucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone. GITR is also expressed in naïve mice by CD25+/CD4+/CD8a- thymocytes and on CD25+/CD4+/CD45RB-low splenocytes. Low levels were detected in splenic CD25+/CD4+/CD45RB-low T cells, B cells, dendritic cells and macrophages. A GITR ligand was detected on dendritic cells, macrophages and B cells.The DTA-1 antibody stimulates GITR and abrogates suppression by T regulatory cells (Treg), without affecting their proliferation. DTA-1 administration or the removal of GITR-expressing cells led to organ specific autoimmune disease.

• NCBI Gene ID:

  21936

• Swiss Prot:

  O35714

• Host:

  Rat

• Reactivity:

  Mouse

• Clonality:

  Monoclonal

• Clone:

  DTA-1

• Conjugation:

  APC

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

• Fragment:

  Rat IgG2b

• Specificity:

  The DTA-1 monoclonal antibody specifically reacts with mouse Glucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone.

• Symbol:

  Tnfrsf18

• NCBI Official Name:

  Tumor necrosis factor receptor superfamily, member 18

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Kataoka, H., Takakura, N., Nishikawa, S., Tsuchida, K., Kodama, H., Kunisada, T., ... Nishikawa, S. I. (1997) . Expressions of PDGF receptor alpha, c‐Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells.Development, growth differentiation,39 (6), 729-740.

• Background Reference 02:

  Ishitobi, H., Matsumoto, K., Azami, T., Itoh, F., Itoh, S., Takahashi, S., Ema, M. (2010) . Flk1-GFP BAC Tg mice: an animal model for the study of blood vessel development.Experimental animals,59 (5), 615-622.

• Background Reference 03:

  Yamashita, J., Itoh, H., Hirashima, M., Ogawa, M., Nishikawa, S., Yurugi, T., ... Nishikawa, S. I. (2000) . Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors.Nature,408 (6808), 92-96.

• Other Product Names:

  AITR, Gitr, Tnfrsf18

• Tested Applications:

  Flow

• Physical Properties:

  Liquid