CD357 Antibody [DTA-1]

• Background:

  The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone. GITR is also expressed in naïve mice by CD25+/CD4+/CD8a- thymocytes and on CD25+/CD4+/CD45RB-low splenocytes. Low levels were detected in splenic CD25+/CD4+/CD45RB-low T cells, B cells, dendritic cells and macrophages. A GITR ligand was detected on dendritic cells, macrophages and B cells.The DTA-1 antibody stimulates GITR and abrogates suppression by T regulatory cells (Treg), without affecting their proliferation. DTA-1 administration or the removal of GITR-expressing cells led to organ specific autoimmune disease.

• NCBI Gene ID:

  21936

• Swiss Prot:

  O35714

• Host:

  Rat

• Reactivity:

  Mouse

• Clonality:

  Monoclonal

• Clone:

  DTA-1

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies, Functional Antibodies

• Field of Research:

  Immunology

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C . Do not freeze.

• Fragment:

  Rat IgG2b

• Specificity:

  The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone.

• Symbol:

  Tnfrsf18

• NCBI Official Name:

  Tumor necrosis factor receptor superfamily, member 18

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Ko, K., Yamazaki, S., Nakamura, K., Nishioka, T., Hirota, K., Yamaguchi, T., ... Sakaguchi, S. (2005) . Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+ CD25+ CD4+ regulatory T cells.The Journal of experimental medicine,202 (7), 885-891.

• Background Reference 02:

  Shimizu, J., Yamazaki, S., Takahashi, T., Ishida, Y., Sakaguchi, S. (2002) . Stimulation of CD25+ CD4+ regulatory T cells through GITR breaks immunological self-tolerance.Nature immunology,3 (2), 135-142.

• Background Reference 03:

  Tone, M., Tone, Y., Adams, E., Yates, S. F., Frewin, M. R., Cobbold, S. P., Waldmann, H. (2003) . Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells.Proceedings of the National Academy of Sciences,100 (25), 15059-15064.

• Other Product Names:

  AITR, Gitr, Tnfrsf18

• Tested Applications:

  Flow, Functional Assays, IHC, IF

• Physical Properties:

  Liquid