CD357 Antibody [DTA-1]

• Background :

  The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone. GITR is also expressed in naïve mice by CD25+/CD4+/CD8a- thymocytes and on CD25+/CD4+/CD45RB-low splenocytes. Low levels were detected in splenic CD25+/CD4+/CD45RB-low T cells, B cells, dendritic cells and macrophages. A GITR ligand was detected on dendritic cells, macrophages and B cells.The DTA-1 antibody stimulates GITR and abrogates suppression by T regulatory cells (Treg), without affecting their proliferation. DTA-1 administration or the removal of GITR-expressing cells led to organ specific autoimmune disease.

• NCBI Gene ID :

  21936

• Swiss Prot :

  O35714

• Host :

  Rat

• Reactivity :

  Mouse

• Clonality :

  Monoclonal

• Clone :

  DTA-1

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies, Functional Antibodies

• Field of Research :

  Immunology

• Purification :

  The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

• Concentration :

  Batch dependent

• Buffer :

  Phosphate-buffered aqueous solution, ph7.2.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  The product should be stored undiluted at 4˚ C . Do not freeze.

• Fragment :

  Rat IgG2b

• Specificity :

  The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone.

• Symbol :

  Tnfrsf18

• NCBI Official Name :

  Tumor necrosis factor receptor superfamily, member 18

• NCBI Organism :

  Mus musculus

• Background Reference 01 :

  Ko, K., Yamazaki, S., Nakamura, K., Nishioka, T., Hirota, K., Yamaguchi, T., ... Sakaguchi, S. (2005) . Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+ CD25+ CD4+ regulatory T cells.The Journal of experimental medicine,202 (7), 885-891.

• Background Reference 02 :

  Shimizu, J., Yamazaki, S., Takahashi, T., Ishida, Y., Sakaguchi, S. (2002) . Stimulation of CD25+ CD4+ regulatory T cells through GITR breaks immunological self-tolerance.Nature immunology,3 (2), 135-142.

• Background Reference 03 :

  Tone, M., Tone, Y., Adams, E., Yates, S. F., Frewin, M. R., Cobbold, S. P., Waldmann, H. (2003) . Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells.Proceedings of the National Academy of Sciences,100 (25), 15059-15064.

• Other Product Names :

  AITR, Gitr, Tnfrsf18

• Tested Applications :

  Flow, Functional Assays, IHC, IF

• Physical Properties :

  Liquid