CD8a Antibody [Hit8a] (APC)

• Background:

  The Hit8a monoclonal antibody reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells. CD8 interacts with the major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. The Hit8a antibody reacts with 13-48% of peripheral lymphocytes, 80% of thymocytes, and a subset of natural killer cells. HIT8a, RPA-T8, and OKT8 antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.

• NCBI Gene ID:

  925

• Swiss Prot:

  P01732

• Host:

  Mouse

• Reactivity:

  Human

• Clonality:

  Monoclonal

• Clone:

  Hit8a

• Conjugation:

  APC

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

• Fragment:

  Mouse IgG1, kappa

• Specificity:

  The Hit8a monoclonal antibody reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells.

• Symbol:

  CD8A

• NCBI Official Name:

  CD8a molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Salem, M. L., Hossain, M. S. (2000) . In vivo acute depletion of CD8+ T cells before murine cytomegalovirus infection upregulated innate antiviral activity of natural killer cells. International journal of immunopharmacology,22 (9), 707-718.

• Background Reference 02:

  Kruisbeek, A. M. (1991) . In Vivo Depletion of CD4 and CD8 Specific T Cells.Current protocols in immunology, 4-1.

• Background Reference 03:

  Davies, A., Kalb, S., Liang, B., Aldrich, C. J., Lemonnier, F. A., Jiang, H., ... Soloski, M. J. (2003) . A peptide from heat shock protein 60 is the dominant peptide bound to Qa-1 in the absence of the MHC class Ia leader sequence peptide Qdm.The Journal of Immunology,170 (10), 5027-5033.

• Other Product Names:

  CD8, MAL, p32, Leu2, CD8A

• Tested Applications:

  Flow

• Physical Properties:

  Liquid