CD40 Antibody [5C3]

• Background:

  The 5C3 monoclonal antibody specifically reacts with human CD40, a 48kDA type I glycoprotein. CD40 is part of the TNFR family and expressed on a subset of T cells, B cells, dendritic cells, and macrophages. It has been shown to be involved in the protection of B cells from apoptosis, B cell differentiation, co-stimulation, and isotype class-switching. The 5C3 antibody partially blocks the binding of CD40L (CD154) . In the presence of IL-4 or PMA, the 5C3 antibody promotes B cell proliferation.

• NCBI Gene ID:

  958

• Swiss Prot:

  P25942

• Host:

  Mouse

• Reactivity:

  Human

• Clonality:

  Monoclonal

• Clone:

  5C3

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies, Functional Antibodies

• Field of Research:

  Immunology

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C . Do not freeze.

• Fragment:

  Mouse IgG1, kappa

• Specificity:

  The 5C3 monoclonal antibody specifically reacts with human CD40, a 48kDA type I glycoprotein.

• Symbol:

  CD40

• NCBI Official Name:

  CD40 molecule, TNF receptor superfamily member 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Schlossman, S. F. (1995) .Leucocyte typing V: White cell differentiation antigens: Proceedings of the Fifth International Workshop and Conference, Held in Boston, USA 3-7 November, 1993. Oxford University Press.

• Background Reference 02:

  Barclay, A. N., Birkeland, M. L., Brown, M. H., Beyers, A. D., David, S. J., Somoza, C., Williams, A. F. The Leukocyte Antigen Facts Book. 1993.Academic Prss, London.

• Background Reference 03:

  Challa, A., Pound, J. D., Gordon, J., Armitage, R. J. (1999) . Epitope‐dependent synergism and antagonism between CD40 antibodies and soluble CD40 ligand for the regulation of CD23 expression and IgE synthesis in human B cells.Allergy,54 (6), 576-583.

• Other Product Names:

  P50, Bp50, CDW40, TNFRSF5, CD40

• Tested Applications:

  Flow, Functional Assays

• Physical Properties:

  Liquid