CD40 Antibody

• Background:

  The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

• NCBI Gene ID:

  958

• Swiss Prot:

  P25942

• Accession Number:

  P25942

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CD40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-276 amino acids from the C-terminal region of human CD40.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Pig

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  31 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CD40

• NCBI Official Name:

  Tumor necrosis factor receptor superfamily member 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rodriguez-Rodriguez, L., et al. J. Rheumatol. 37 (10) :2076-2080 (2010)

• Background Reference 02:

  Lewis, J.P., et al. Genomics 96 (4) :211-219 (2010)

• Background Reference 03:

  Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

• Background Reference 04:

  Blanco-Kelly, F., et al. PLoS ONE (7), E11520 (2010) :

• Other Product Names:

  Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD40, CD40, TNFRSF5

• Tested Applications:

  WB

• Protein ID:

  116000

• Physical Properties:

  Liquid