Syk (Ab 323) Antibody

• Background:

  Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C γ dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion

• NCBI Gene ID:

  6850

• Swiss Prot:

  P43405

• Accession Number:

  NP_001128524.1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Syk (Ab-323) antibody was raised against a peptide sequence around aa. 321~325 (N-P-Y-E-P) derived from Human syk.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  72 kDa

• Specificity:

  This antibody detects endogenous level of total syk protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  SYK

• NCBI Official Name:

  Spleen tyrosine kinase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447. Turner, M. et al. (2000) Immunol. Today 21, 148-154. Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874. Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.

• Other Product Names:

  P72-Syk, IKBE, Spleen tyrosine kinase

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  205277435

• Physical Properties:

  Liquid