Raf1 (Ab 338) Antibody

• Background:

  Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

• NCBI Gene ID:

  5894

• Swiss Prot:

  P04049

• Accession Number:

  NP_002871.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Raf1 (Ab-338) antibody was raised against a peptide sequence around aa.336~340 (R-D-S-S-Y) derived from Human RAF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  73 kDa

• Specificity:

  This antibody detects endogenous level of total Raf1 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  RAF1

• NCBI Official Name:

  V-raf-1 murine leukemia viral oncogene homolog 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Adams DG, et al. (2005) J Biol Chem. 280 (52) : 42644-54. Miura S, et al. (2003) Biochem Biophys Res Commun. 306 (4) : 924-9. Noser JA, et al. (2007) Mol Ther. 15 (8) : 1531-6.

• Other Product Names:

  NS5, CRAF, Raf-1, c-Raf, ESR, NR3A1, C-RAF, C-Raf

• Tested Applications:

  WB, IHC

• Protein ID:

  4506401

• Physical Properties:

  Liquid