Cyclin O Antibody

• Background:

  Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.

• NCBI Gene ID:

  10309

• Swiss Prot:

  P22674

• Accession Number:

  NP_066970

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  Cyclin O Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1410 - Mouse Bladder Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  Cyclin O Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Cyclin O antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  39 kDa

• Fragment:

  IgG

• Specificity:

  At least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms.

• Symbol:

  CCNO

• NCBI Official Name:

  Cyclin O

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Roig MB, Roset R, Ortet L, et al. Identification of a novel cyclin required for the intrinsic apoptosis pathway in lymphoid cells. Cell Death Diff. 2009; 16:230-43.

• Background Reference 02:

  Muller SJ and Caradonna S. Isolation and characterization of a human cDNA encoding uracil-DNA glycosylase. Biochim. Biophys. Acta 1991; 1088:197-207.

• Background Reference 03:

  Muller SJ and Caradonna S. Cell cycle regulation of a human cyclin-like gene encoding uracil-DNA glycosylase. J. Biol. Chem. 1993; 268:1310-9

• Other Product Names:

  Cyclin O Antibody: CCNU, UDG2, Cyclin-O

• Tested Applications:

  ELISA, WB

• Protein ID:

  153791755

• Physical Properties:

  Liquid