4E BP1 (phospho Thr45) Antibody

• Background:

  4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

• NCBI Gene ID:

  1978

• Swiss Prot:

  Q13541

• Accession Number:

  NP_004086.1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  4E-BP1 (Phospho-Thr45) antibody was raised against a peptide sequence around phosphorylation site of threonine 45 (S-T-T (p) -P-G) derived from Human 4E-BP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  18 kDa

• Specificity:

  This antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  EIF4EBP1

• NCBI Official Name:

  Eukaryotic translation initiation factor 4E binding protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gingras AC, et al. (1998) Genes Dev 12 (4) : 502-513. Brugarolas J, et al. (2004) Genes Dev 18 (23) : 2893-2904. Kumar V, et al. (2000) EMBO J 19 (5) : 1087-1097. Moody CA, et al. (2005) J Virol 79 (9) : 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95 (4) : 1432-1437.

• Other Product Names:

  BP-1, 4EBP1, 4E-BP1, PHAS-I, STK14A, EIF4EBP1, PHAS-1

• Tested Applications:

  WB, IHC

• Protein ID:

  4758258

• Physical Properties:

  Liquid