Myc (phospho Thr58) Antibody

• Background:

  Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

• NCBI Gene ID:

  4609

• Swiss Prot:

  P01106

• Accession Number:

  NP_002458.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Myc (Phospho-Thr58) antibody was raised against a peptide sequence around phosphorylation site of threonine 58 (L-P-T (p) -P-P) derived from Human Myc.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  60 kDa

• Specificity:

  This antibody detects endogenous level of Myc only when phosphorylated at threonine 58.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  MYC

• NCBI Official Name:

  V-myc avian myelocytomatosis viral oncogene homolog

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jin Z, et al. (2004) J Biol Chem. 279 (38) : 40209-40219. Welcker M, et al. (2004) Proc Natl Acad Sci U S A. 101 (24) : 9085-9090. Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702. Blackwood E M, et al. (1991) Science. 251:1211-1217. Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.

• Other Product Names:

  MRTL, MYCC, c-Myc, bHLHe39, BBC6, BCL2L8, c-myc

• Tested Applications:

  WB, IHC

• Protein ID:

  71774083

• Physical Properties:

  Liquid