Akt (phospho Ser473) Antibody

• Background:

  General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

• NCBI Gene ID:

  207

• Swiss Prot:

  P31749

• Accession Number:

  NP_001014431.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Akt (Phospho-Ser473) antibody was raised against a peptide sequence around phosphorylation site of serine 473 (Q-F-S (p) -Y-S) derived from Human Akt.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  60 kDa

• Specificity:

  This antibody detects endogenous level of Akt only when phosphorylated at serine 473.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  AKT1

• NCBI Official Name:

  V-akt murine thymoma viral oncogene homolog 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Baudhuin LM, et al. (2004) FASEB J Feb; 18 (2) : 341-3. Min YH, et al. (2004) Cancer Res; 64 (15) : 5225-31. Feng J, et al. (2004) J Biol Chem; 279 (34) : 35510-7.

• Other Product Names:

  AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, NFKB3, C-AKT, PKB-alpha, RAC-PK-alpha

• Tested Applications:

  WB, IHC

• Protein ID:

  62241013

• Physical Properties:

  Liquid