GSK3a (phospho Ser21) Antibody

• Background:

  Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.

• NCBI Gene ID:

  2931

• Swiss Prot:

  P49840

• Accession Number:

  NP_063937.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  GSK3a (Phospho-Ser21) antibody was raised against a peptide sequence around phosphorylation site of serine 21 (T-S-S (p) -F-A) derived from Human GSK3α .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  51 kDa

• Specificity:

  This antibody detects endogenous level of GSK3α only when phosphorylated at serine 21and does not detect GSK-3β when phosphorylated at Ser9.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  GSK3A

• NCBI Official Name:

  Glycogen synthase kinase 3 alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Barry FA, et al. (2003) FEBS Lett. 553 (1-2) : 173-178. Koivisto L, et al. (2003) J Cell Sci. 116 (Pt 18) : 3749-3760. Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.

• Other Product Names:

  MAPTL, MTBT1, TAU, Factor A, GSK-3 alpha, kinase GSK3-alpha

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  49574532

• Physical Properties:

  Liquid