NEK1 Antibody

• Background:

  Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K) . MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

• NCBI Gene ID:

  4750

• Swiss Prot:

  Q96PY6

• Accession Number:

  Q96PY6

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1165-1196 amino acids from the C-terminal region of human NEK1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  143 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  NEK1

• NCBI Official Name:

  Serine/threonine-protein kinase Nek1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Surpili, M.J., et al., Biochemistry 42 (51) :15369-15376 (2003) .

• Background Reference 02:

  Scanlan, M.J., et al., Int. J. Cancer 83 (4) :456-464 (1999) .

• Background Reference 03:

  Letwin, K., et al., EMBO J. 11 (10) :3521-3531 (1992) .

• Other Product Names:

  Serine/threonine-protein kinase Nek1, Never in mitosis A-related kinase 1, NimA-related protein kinase 1, Renal carcinoma antigen NY-REN-55, NEK1, KIAA1901

• Tested Applications:

  WB, IHC-P

• Protein ID:

  22256934

• Physical Properties:

  Liquid