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NEK3 Antibody

CAT: 0223-26-375Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-26-375Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
NEK3 is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. It differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase.In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized.
NCBI Gene ID
4752
Swiss Prot
P51956
Accession Number
NP_002489
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NEK3.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle, Cancer, Signal Transduction
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NEK3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
58 kDa
Applications Notes
NEK3 antibody can be used for detection of NEK3 by ELISA at 1:1562500. NEK3 antibody can be used for detection of NEK3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
NEK3
NCBI Official Name
NIMA-related kinase 3
NCBI Organism
Homo sapiens
Background Reference 01
Miller, S.L., (2007) Oncogene 26 (32), 4668-4678.
Other Product Names
NEK3, HSPK36, MGC29949
Tested Applications
ELISA, WB
Protein ID
23510391
Physical Properties
Liquid

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