NEK3 Antibody

• Background:

  In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. NEK3 is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm.

• NCBI Gene ID:

  4752

• Swiss Prot:

  P51956

• Accession Number:

  P51956

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NEK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the Central region of human NEK3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  58 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:2000

• Symbol:

  NEK3

• NCBI Official Name:

  Serine/threonine-protein kinase Nek3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Schultz, S.J., et al., Cell Growth Differ. 5 (6) :625-635 (1994) .

• Background Reference 02:

  Schultz, S.J., et al., Cell Growth Differ. 4 (10) :821-830 (1993) .

• Background Reference 03:

  Kimura, M., et al., Cytogenet. Cell Genet. 95 (3-4), 177-182 (2001) .

• Other Product Names:

  Serine/threonine-protein kinase Nek3, HSPK 36, Never in mitosis A-related kinase 3, NimA-related protein kinase 3, NEK3

• Tested Applications:

  WB

• Protein ID:

  20178297

• Physical Properties:

  Liquid