MLCK Antibody

CAT: 0223-63-313Size: 400 µLDry Ice: NoHazardous: No
CAT#:0223-63-313Size:400 µL
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
NCBI Gene ID
4638
Swiss Prot
Q15746
Accession Number
Q15746
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
This MLCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 908-938 amino acids from the N-terminal region of human MLCK.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Signal Transduction
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Rb
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
211 kDa
Fragment
Rabbit Ig
Symbol
MYLK
NCBI Official Name
Myosin light chain kinase, smooth muscle
NCBI Organism
Homo sapiens
Background Reference 01
Lazar, V., et al., Genomics 57 (2) :256-267 (1999) . Watterson, D.M., et al., J. Cell. Biochem. 75 (3) :481-491 (1999) . Garcia, J.G., et al., Am. J. Respir. Cell Mol. Biol. 16 (5) :489-494 (1997) . Potier, M.C., et al., Genomics 29 (3) :562-570 (1995) .
Other Product Names
Myosin light chain kinase, smooth muscle, MLCK, smMLCK, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK, MLCK, MLCK1, MYLK1
Tested Applications
WB, IHC-P
Protein ID
300669714
Physical Properties
Liquid

Related Products

CatalogName
60-229MLC1 Antibody
43-286MLC1 Antibody
43-286PMLC1 Peptide
64-160WAS Antibody
13-349WAS Antibody
60-596WAS Antibody
63-408DGKB Antibody
63-412AK5 Antibody
63-420HK3 Antibody
63-422HK2 Antibody

Alternative Products

CatalogName
DF4719-01MLCK Antibody
MBS9455900-01MLCK Conjugated Antibody
bs-4705RMLCK Polyclonal Antibody
abx217014MLCK (pY471) Antibody
MBS9614388-01Phospho-MLCK (Tyr471) Antibody
TMAB-08843-01Anti-MLCK Polyclonal Antibody
R1510-18MLCK Rabbit Polyclonal Antibody
E45R14359G-4MLCK Antibody (N-term)
SL4705R-01Rabbit Anti-MLCK antibody
12692-01MLCK (Phospho-Tyr471) Antibody

Popular Products