MLCK Antibody

• Background:

  MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

• NCBI Gene ID:

  4638

• Swiss Prot:

  Q15746

• Accession Number:

  Q15746

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This MLCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 908-938 amino acids from the N-terminal region of human MLCK.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rb

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  211 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  MYLK

• NCBI Official Name:

  Myosin light chain kinase, smooth muscle

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lazar, V., et al., Genomics 57 (2) :256-267 (1999) . Watterson, D.M., et al., J. Cell. Biochem. 75 (3) :481-491 (1999) . Garcia, J.G., et al., Am. J. Respir. Cell Mol. Biol. 16 (5) :489-494 (1997) . Potier, M.C., et al., Genomics 29 (3) :562-570 (1995) .

• Other Product Names:

  Myosin light chain kinase, smooth muscle, MLCK, smMLCK, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK, MLCK, MLCK1, MYLK1

• Tested Applications:

  WB, IHC-P

• Protein ID:

  300669714

• Physical Properties:

  Liquid