MLCK Rabbit Polyclonal Antibody

CAT: 0992-R1510-18Size: 100 µLDry Ice: NoHazardous: No
CAT#:0992-R1510-18Size:100 µL
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Description
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and myosin light chain kinases (MYLK, also designated MLCK). The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity. Non-muscle, smooth muscle and skeletal/cardiac muscle MYLK isoforms exist. The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments. Multiple transcript variants are described for the MYLK gene. Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues. The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, MYLK2 (also designated skMLCK). MYLK appears to be a target for PAKs (p21-activated kinases). PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation.
CAS Number
9007-83-4
Product Name Alternative
deglutamylated form antibody DKFZp686I10125 antibody EC 2.7.11.18 antibody FLJ12216 antibody Kinase related protein antibody Kinase-related protein antibody KRP antibody MLCK antibody MLCK1 antibody MLCK108 antibody MLCK210 antibody MSTP083 antibody MYLK antibody MYLK_HUMAN antibody MYLK1 antibody Myosin light chain kinase antibody Myosin light polypeptide kinase antibody OTTHUMP00000180642 antibody OTTHUMP00000180643 antibody smMLCK antibody smooth muscle antibody Smooth muscle myosin light chain kinase antibody Telokin antibody
Abbreviation
MLCK,smMLCK,KRP
Swiss Prot
Q15746 Human
Cellular Locus
Cytoplasm, Cleavage furrow, Cell projection
Host
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Synthetic peptide within Human MLCK aa 36-85 / 1,914.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
IF-Cell, IHC-P, FC
Positive Control
Hela, NIH/3T3, SHG-44, mouse smooth muscle tissue.
Concentration
1 mg/mL
Dilution
IF-Cell: 1:50-1:200;IF-Cell: 1:50-1:200;FC: 1:50-1:100
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No

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