PAK1 Antibody

• Background:

  PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.

• NCBI Gene ID:

  5058

• Swiss Prot:

  Q13153

• Accession Number:

  Q13153

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from human PAK1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat, Rabbit, Xenopus

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  61 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  PAK1

• NCBI Official Name:

  Serine/threonine-protein kinase PAK 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chen, S., et al., J. Biol. Chem. 278 (22) :20029-20036 (2003) .

• Background Reference 02:

  Sells, M.A., et al., Curr. Biol. 7 (3) :202-210 (1997) .

• Background Reference 03:

  Brown, J.L., et al., Curr. Biol. 6 (5) :598-605 (1996) .

• Other Product Names:

  Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK, PAK1

• Tested Applications:

  WB, IHC-P, IF

• Protein ID:

  90111767

• Physical Properties:

  Liquid