ACTN4 Antibody

• Background:

  Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This protein is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and is thought to be involved in metastatic processes. Mutations in the gene encoding this protein have been associated with focal and segmental glomerulosclerosis.

• NCBI Gene ID:

  81

• Swiss Prot:

  O43707

• Accession Number:

  O43707

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ACTN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 785-813 amino acids from the C-terminal region of human ACTN4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  105 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  ACTN4

• NCBI Official Name:

  Alpha-actinin-4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kikuchi, S., Clin. Cancer Res. 14 (17), 5348-5356 (2008)

• Background Reference 02:

  Barbolina, M.V., Lab. Invest. 88 (6), 602-614 (2008)

• Background Reference 03:

  Kimura, M., Intern. Med. 47 (12), 1099-1106 (2008)

• Other Product Names:

  Alpha-actinin-4, F-actin cross-linking protein, Non-muscle alpha-actinin 4, ACTN4

• Tested Applications:

  WB, IHC-P, Flow

• Physical Properties:

  Liquid