ACTN2 Antibody

• Background:

  Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.

• NCBI Gene ID:

  88

• Swiss Prot:

  P35609

• Accession Number:

  P35609

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This ACTN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 751-779 amino acids from the C-terminal region of human ACTN2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  104 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  ACTN2

• NCBI Official Name:

  Alpha-actinin-2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Saus, E., et al.Psychiatr Res 44 (14) :971-978 (2010)

• Background Reference 02:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 03:

  Zimmerman, R.S., et al. Genet. Med. 12 (5) :268-278 (2010)

• Background Reference 04:

  Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34 (5) :800-812 (2010)

• Other Product Names:

  Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2

• Tested Applications:

  WB

• Protein ID:

  543742

• Physical Properties:

  Liquid