DOK2 Antibody

• Background:

  Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. Dok-2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. Dok-2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung. Expression of bcr/abl induces additional tyrosine phosphorylation of the Dok1 and Dok2 proteins and their association with Ras-GAP. Thus, it is suspected that DOK association regulates GAP activity toward Ras and that the Dok proteins serve as mediators of bcr-abl signaling. The role of Dok proteins in bcr-abl regulation may also be implicated in chronic myelogenous leukemia (CML), which is characterized by a Philadelphia chromosome translocation t (9;22) . Such a mutation would result in a p210-bcr/abl chimeric protein-tyrosine kinase which has been found in many CML cases.

• NCBI Gene ID:

  9046

• Swiss Prot:

  O60496

• Accession Number:

  O60496

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This DOK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-412 amino acids from the C-terminal region of human DOK2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  45 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  DOK2

• NCBI Official Name:

  Docking protein 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Salomon, A.R., et al., Proc. Natl. Acad. Sci. U.S.A. 100 (2) :443-448 (2003) .

• Background Reference 02:

  Di Cristofano, A., et al., J. Biol. Chem. 273 (9) :4827-4830 (1998) .

• Other Product Names:

  Docking protein 2, Downstream of tyrosine kinase 2, p56 (dok-2), DOK2

• Tested Applications:

  WB, IHC-P

• Physical Properties:

  Liquid