HARS Antibody

• Background:

  Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. This enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The protein is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

• NCBI Gene ID:

  3035

• Swiss Prot:

  P12081

• Accession Number:

  P12081

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the N-terminal region of human HARS.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  57 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  HARS

• NCBI Official Name:

  Histidine--tRNA ligase, cytoplasmic

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Levine, S.M., Arthritis Rheum. 56 (8), 2729-2739 (2007)

• Background Reference 02:

  Lu, Q., Proc. Natl. Acad. Sci. U.S.A. 100 (13), 7626-7631 (2003)

• Other Product Names:

  Histidine--tRNA ligase, cytoplasmic, Histidyl-tRNA synthetase, HisRS, HARS, HRS

• Tested Applications:

  WB, IHC-P

• Protein ID:

  135123

• Physical Properties:

  Liquid