NLK Antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K) . MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

51701

Q9UBE8

Q9UBE8

Human

This NLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-436 amino acids from the C-terminal region of human NLK.

Unconjugated

Primary Antibodies

Signal Transduction

This antibody is purified through a protein A column, followed by peptide affinity purification.

Batch dependent

Predicted species reactivity based on immunogen sequence: Mouse, Rat, Bovine

Supplied in PBS with 0.09% (W/V) sodium azide.

None

Blue Ice

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

58 kDa

Rabbit Ig

NLK

Serine/threonine-protein kinase NLK

Homo sapiens

Yasuda, J., et al., Biochem. Biophys. Res. Commun. 308 (2) :227-233 (2003) .

Ishitani, T., et al., Mol. Cell. Biol. 23 (4) :1379-1389 (2003) .

Ishitani, T., et al., Mol. Cell. Biol. 23 (1) :131-139 (2003) .

Kehrer-Sawatzki, H., et al., Gene 251 (1) :63-71 (2000) .

Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1, NLK, LAK1 {ECO:0000312|EMBL:AAD560131}

WB, IHC-P

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