MGMT Antibody

• Background:

  MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. This protein repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the protein is irreversibly inactivated.

• NCBI Gene ID:

  4255

• Swiss Prot:

  P16455

• Accession Number:

  P16455

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-182 amino acids from the C-terminal region of human MGMT.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  22 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  MGMT

• NCBI Official Name:

  Methylated-DNA--protein-cysteine methyltransferase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kim, J.I., Suh, J.T. Hum. Pathol. 40 (7), 934-941 (2009)

• Background Reference 02:

  Chen, S.P., Chiu, S.C. Genet Test Mol Biomarkers 13 (1), 67-71 (2009)

• Background Reference 03:

  Slupphaug, G., Lettrem, I. Carcinogenesis 13 (10), 1769-1773 (1992)

• Background Reference 04:

  Lee, S.M., Crowther, D. Br. J. Cancer 66 (2), 331-336 (1992)

• Other Product Names:

  Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT

• Tested Applications:

  WB, IHC-P, Flow

• Protein ID:

  127069

• Physical Properties:

  Liquid