HSP27 (phospho Ser15) Antibody

• Background:

  Involved in stress resistance and actin organization.

• NCBI Gene ID:

  3315

• Swiss Prot:

  P04792

• Accession Number:

  NP_001531.1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  HSP27 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine15 (G-P-S (p) -W-D) derived from Human HSP27.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  27 kDa

• Specificity:

  This antibody detects endogenous level of HSP27only when phosphorylated at serine 15.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  HSPB1

• NCBI Official Name:

  Heat shock 27kDa protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Tremolada L, et al. (2005) Proteomics. 5 (3) : 788-795. McGregor E, et al. (2004) Mol Cell Proteomics. 3 (2) : 115-124. Benn SC, et al. (2002) Neuron. 36 (1) : 45-56. MacDonald JA, et al. (2002) Mol Cell Proteomics. 1 (4) : 314-322.

• Other Product Names:

  CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, ASPL, RCC17, TUG, UBXD9, UBXN9, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  4504517

• Physical Properties:

  Liquid