NAGK Antibody

• Background:

  N-acetylglucosamine kinase (NAGK) converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. NAGK belongs to the group of N-acetylhexosamine kinases and is a prominent salvage enzyme of amino sugar metabolism in mammals. The predicted 344-amino acid NAGK protein contains the 5 sequence motifs necessary for the binding of ATP by sugar kinases. NAGK shares 91.6% amino acid similarity with mouse Nagk, for which enzyme activity is detectable in all mouse tissues examined, with highest enzymatic activity in testis. It is hypothesized that NAGK has a general role in the catabolic pathways of GlcNAc as well as of ManNAc.

• NCBI Gene ID:

  55577

• Swiss Prot:

  Q9UJ70

• Accession Number:

  Q9UJ70

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This NAGK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-330 amino acids from the C-terminal region of human NAGK.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Obesity, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  37 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  NAGK

• NCBI Official Name:

  N-acetyl-D-glucosamine kinase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hinderlich, S., et al., Eur. J. Biochem. 267 (11) :3301-3308 (2000) .

• Background Reference 02:

  Lowes, W., et al., Biochim. Biophys. Acta 1379 (1) :134-142 (1998) .

• Background Reference 03:

  Weidanz, J.A., et al., Br. J. Haematol. 95 (4) :645-653 (1996) .

• Other Product Names:

  N-acetyl-D-glucosamine kinase, N-acetylglucosamine kinase, GlcNAc kinase, NAGK

• Tested Applications:

  WB

• Protein ID:

  24638065

• Physical Properties:

  Liquid