GRK4 Antibody

• Background:

  Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK) .

• NCBI Gene ID:

  2868

• Swiss Prot:

  P32298

• Accession Number:

  P32298

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This GRK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-577 amino acids from the C-terminal region of human GRK4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  67 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  GRK4

• NCBI Official Name:

  G protein-coupled receptor kinase 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Perroy, J., et al., EMBO J. 22 (15) :3816-3824 (2003) .

• Background Reference 02:

  Watanabe, H., et al., Kidney Int. 62 (3) :790-798 (2002) .

• Background Reference 03:

  Sallese, M., et al., J. Biol. Chem. 272 (15) :10188-10195 (1997) .

• Background Reference 04:

  Premont, R.T., et al., J. Biol. Chem. 271 (11) :6403-6410 (1996) .

• Other Product Names:

  G protein-coupled receptor kinase 4, G protein-coupled receptor kinase GRK4, ITI1, GRK4, GPRK2L, GPRK4

• Tested Applications:

  WB, IHC-P

• Protein ID:

  143811400

• Physical Properties:

  Liquid