Aurora-A Antibody

• Background:

  Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Auroria kinase A may play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. It may be involved in microtubule formation and/or stabilization. This protein has also been postulated to play a key role during tumor development and progression. Aurora kinase A localizes on centrosomes in interphase cells and at each spindle pole in mitosis. It is highly expressed in testis, weakly in skeletal muscle, thymus and spleen, and also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly afterward. Defects in Aurora kinase A are responsible for numerical centrosome aberrations including aneuploidy.

• NCBI Gene ID:

  6790

• Swiss Prot:

  O14965

• Accession Number:

  O14965

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Aurora-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human Aurora-A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  46 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  AURKA

• NCBI Official Name:

  Aurora kinase A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26) :16899-16903 (2002) . Tanaka, M., et al., J. Biol. Chem. 277 (12) :10719-10726 (2002) . Nigg, E.A., Nat. Rev. Mol. Cell Biol. 2 (1) :21-32 (2001) . Deloukas, P., et al., Nature 414 (6866) :865-871 (2001) . Shindo, M., et al., Biochem. Biophys. Res. Commun. 244 (1) :285-292 (1998) .

• Other Product Names:

  Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, hARK1, Breast tumor-amplified kinase, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, AURKA

• Tested Applications:

  WB, IF, Flow

• Physical Properties:

  Liquid