WISP1 Antibody

• Background:

  Wisp1 is a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. Wisp1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level.

• NCBI Gene ID:

  8840

• Swiss Prot:

  O95388

• Accession Number:

  O95388

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This WISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human WISP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  40 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  WISP1

• NCBI Official Name:

  WNT1-inducible-signaling pathway protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hocevar, B.A., et al., EMBO J. 22 (12) :3084-3094 (2003) .

• Background Reference 02:

  Tanaka, S., et al., Hepatology 37 (5) :1122-1129 (2003) .

• Background Reference 03:

  Soon, L.L., et al., J. Biol. Chem. 278 (13) :11465-11470 (2003) .

• Background Reference 04:

  Su, F., et al., Genes Dev. 16 (1) :46-57 (2002) .

• Other Product Names:

  WNT1-inducible-signaling pathway protein 1, WISP-1, CCN family member 4, Wnt-1-induced secreted protein, WISP1, CCN4

• Tested Applications:

  WB, IHC-P

• Physical Properties:

  Liquid