TAP1 Antibody

• Background:

  TAP is an integral transmembrane protein involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. TAP is inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. It is inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP and prevents the conformational rearrangement of TAP induced by peptide binding. TAP is also inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP is down-regulated by human Epstein-barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. TAP1 and TAP2 form a heterodimer of TAP1 and TAP2, and the peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2. TAP, inducible by interferon gamma, belongs to the ABC transporter family, MDR subfamily.

• NCBI Gene ID:

  6890

• Swiss Prot:

  Q03518

• Accession Number:

  Q03518

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This TAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 765-794 amino acids from the C-terminal region of human TAP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  87 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  TAP1

• NCBI Official Name:

  Antigen peptide transporter 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lajoie, J., et al., Hum. Immunol. 64 (8) :823-829 (2003) .

• Background Reference 02:

  Gaudet, R., et al., EMBO J. 20 (17) :4964-4972 (2001) .

• Background Reference 03:

  Tang, J., et al., Hum. Immunol. 62 (3) :256-268 (2001) .

• Background Reference 04:

  Hewitt, E.W., et al., EMBO J. 20 (3) :387-396 (2001) .

• Other Product Names:

  Antigen peptide transporter 1, APT1, ATP-binding cassette sub-family B member 2, Peptide supply factor 1, Peptide transporter PSF1, PSF-1, Peptide transporter TAP1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 protein, TAP1, ABCB2, PSF1, RING4, Y3

• Tested Applications:

  WB, IHC-P

• Protein ID:

  215273957

• Physical Properties:

  Liquid