Cry1 Antibody

• Background:

  Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central ?gears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

• NCBI Gene ID:

  1407

• Swiss Prot:

  Q16526

• Accession Number:

  Q16526

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Cry1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 556-586 amino acids from the C-terminal region of human Cry1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Monkey

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  66 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  CRY1

• NCBI Official Name:

  Cryptochrome-1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Griffin, E.A. Jr., et al., Science 286 (5440) :768-771 (1999) .

• Background Reference 02:

  Van der Horst, G.T., et al., Nature 398 (6728) :627-630 (1999) .

• Background Reference 03:

  Kobayashi, K., et al., Nucleic Acids Res. 26 (22) :5086-5092 (1998) .

• Background Reference 04:

  Hsu, D.S., et al., Biochemistry 35 (44) :13871-13877 (1996) .

• Other Product Names:

  Cryptochrome-1, CRY1, PHLL1

• Tested Applications:

  WB, IHC-P

• Protein ID:

  74735764

• Physical Properties:

  Liquid