FEN1 Antibody

• Background:

  FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. This protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

• NCBI Gene ID:

  2237

• Swiss Prot:

  P39748

• Accession Number:

  P39748

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This FEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the Central region of human FEN1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  43 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  FEN1

• NCBI Official Name:

  Flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hiraoka L.R., Harrington J.J., Gerhard D.S., Genomics 25:220-225 (1995)

• Background Reference 02:

  Robins P., Pappin D.J.C., Wood R.D., Lindahl T.J. Biol. Chem. 269:28535-28538 (1994)

• Background Reference 03:

  Gary R., Ludwig D.L., Cornelius H.L., MacInnes M.A., J. Biol. Chem. 272:24522-24529 (1997)

• Other Product Names:

  Flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}, FEN-1 {ECO:0000255|HAMAP-Rule:MF_03140}, 31-- {ECO:0000255|HAMAP-Rule:MF_03140}, DNase IV, Flap structure-specific endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}, Maturation factor 1, MF1, hFEN-1, FEN1 {ECO:0000255|HAMAP-Rule:MF_03140}, RAD2

• Tested Applications:

  WB, IF

• Protein ID:

  729475

• Physical Properties:

  Liquid